1 1 2 6 S - 1 RNA function leads to a delay in sporulation in B
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چکیده
6S-1 RNA function leads to a delay in sporulation in B. subtilis 3 4 5 6 Amy T. Cavanagh and Karen M. Wassarman# 7 8 9 10 Department of Bacteriology 11 University of Wisconsin-Madison 12 1550 Linden Dr. 13 Madison, WI 53706 14 15 16 Running Title: 6S-1 RNA function delays sporulation in B. subtilis 17 18
منابع مشابه
6S-1 RNA function leads to a delay in sporulation in Bacillus subtilis.
We have discovered that 6S-1 RNA (encoded by bsrA) is important for appropriate timing of sporulation in Bacillus subtilis in that cells lacking 6S-1 RNA sporulate earlier than wild-type cells. The time to generate a mature spore once the decision to sporulate has been made is unaffected by 6S-1 RNA, and, therefore, we propose that it is the timing of onset of sporulation that is altered. Inter...
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The kinetics of deoxyribonucleic acid (DNA), ribonucleic acid (RNA), and protein synthesis as well as protein breakdown during sporulation by Clostridium perfringens were determined. Maximum levels of DNA and net RNA synthesis occurred 3 and 2 h, respectively, after inoculation of sporulation medium. The rate of RNA synthesis decreased as sporulation progressed. Deoxyadenosine increased uptake ...
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The stability of postlogarithmic mRNA has been assessed in Bacillus subtilis during sporulation time periods. The appearance of refractile bodies, alkaline phosphatase synthesis, and total protein synthesis is controlled by mRNA which has an estimated half-life of less than 10 min. Inhibition of RNA synthesis at various times by rifampin addition or temperature shift with a temperature-sensitiv...
متن کاملThe Stability of Messenger Kibonucleic Acid during Sporulation in Bacillus subtiZis*
The stability of postlogarithmic mRNA has been assessed in Bacillus subtilis during sporulation time periods. The appearance of refractile bodies, alkaline phosphatase synthesis, and total protein synthesis is controlled by mRNA which has an estimated half-life of less than 10 min. Inhibition of RNA synthesis at various times by rifampin addition or temperature shift with a temperature-sensitiv...
متن کاملBacillus subtilis aconitase is required for efficient late-sporulation gene expression.
Bacillus subtilis aconitase, encoded by the citB gene, is homologous to the bifunctional eukaryotic protein IRP-1 (iron regulatory protein 1). Like IRP-1, B. subtilis aconitase is both an enzyme and an RNA binding protein. In an attempt to separate the two activities of aconitase, the C-terminal region of the B. subtilis citB gene product was mutagenized. The resulting strain had high catalytic...
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تاریخ انتشار 2013